PolyLC HPLC Columns in India
Why Choose PolyLC Columns
PolyLC Column Lineup (India)
Quick introduction to the commonly requested PolyLC chemistries used for proteins, peptides, glycans, and nucleic acids.
PolyCAT A
Strong cation‑exchange for proteins — widely used for variant profiling (deamidation, PEGylation, desialylation), hemoglobin variants, and monoclonal antibodies. Best suited for proteins with higher pI values.
PolyETHYL A
Hydrophobic interaction chromatography (HIC) for proteins and peptides. Useful for purification workflows and for differentiating very small polarity changes in proteins, including antibody characterization.
PolyHYDROXYETHYL A
HILIC phase that can also run as SEC in fully aqueous conditions. Common for polar small‑molecule work, peptide mapping, complex digest fractionation, oligonucleotides, and sample clean‑up (detergents/lipids/salts).
PolySULFOETHYL A
Cation‑exchange designed for peptides. Popular in multidimensional proteomics (tryptic digests), synthetic peptide QC/purification, and selective isolation of phosphopeptides, disulfide‑linked peptides, and C‑terminal fragments.
PolyMETHYL A
HIC option for proteins and peptides. Fits purification schemes (ion‑exchange → salt addition → HIC) and supports antibody characterization and QC of subtle positional/modified variants.
PolyWAX LP
Anion‑exchange for proteins and nucleic acids. Used for acidic proteins/polypeptides, oligonucleotides and PCR products, mixed‑bed predigest fractionation in proteomics, and small acidic solutes.
PolyGLYCOPLEX A
Neutral, high‑capacity HILIC material for complex carbohydrates/glycans. Often runs with acetonitrile/water (MS‑friendly). Works well for native glycans and labeled derivatives, including sialylated/asialo species.
PolyPROPYL A
HIC phase for proteins and peptides — a strong choice for multidimensional purification, polypeptide purification (e.g., venoms/glycopeptides), antibody characterization, and QC of small polarity differences.
PolyLC Column Part Numbers & Specifications
Below is a master table for all PolyLC columns. Replace the TBD fields with your exact ordering codes (part numbers) and specifications.
| Column | Part No. | Dimensions | Particle Size | Pore Size | pH Range | Max Temp | Max Pressure | Formats | Notes / Typical Use |
|---|---|---|---|---|---|---|---|---|---|
| PolyCAT™ A SCX (Proteins) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical/Prep (TBD) | Charge-variant profiling, mAbs, hemoglobin variants. |
| PolyETHYL™ A HIC (Proteins/Peptides) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical/Prep (TBD) | Non-denaturing HIC; antibody characterization. |
| PolyHYDROXYETHYL™ A HILIC / aqueous SEC-mode |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical (TBD) | Peptide digests, oligos, polar metabolites; MS-friendly. |
| PolySULFOETHYL™ A Peptide SCX (Proteomics) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical (TBD) | 2D-LC workflows; peptide QC; PTM separations. |
| PolyMETHYL™ A HIC (Fine selectivity) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical/Prep (TBD) | Subtle hydrophobic variants; stability/comparability. |
| PolyWAX™ LP WAX (Proteins/Nucleic acids) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical (TBD) | Oligos/PCR; acidic proteins; prefractionation. |
| PolyGLYCOPLEX™ A Glycan HILIC |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical (TBD) | Native/labeled glycans; glycosylation profiling; FL/MS. |
| PolyPROPYL™ A HIC (Stronger retention) |
TBD | TBD | TBD | TBD | TBD | TBD | TBD | Analytical/Prep (TBD) | Complex mixtures; enhanced HIC resolution window. |
Frequently Asked Questions
Which PolyLC column should I choose for proteins?
For protein charge variants, start with PolyCAT™ A (SCX). For non-denaturing hydrophobic profiling/purification, consider PolyETHYL™ A or PolyMETHYL™ A (HIC). Share your protein type, pI, and detector so we can recommend the best phase and starting method.
Do you supply PolyLC columns in India?
Yes. PolyLC Columns Supplier (Akira Analytical Solutions Pvt Ltd) supports genuine PolyLC sourcing in India with quotations, lead times, and application guidance.
How do I request a quotation?
Send the column name, dimensions, particle size, and quantity using the Contact page. We will reply with availability, price, and delivery timeline.