PolyLC Column Range

PolyCAT A is a dedicated strong cation‑exchange phase built for demanding separations of proteins and other large biomolecules. Its polymeric base and permanently charged sulfonic acid functionality provide stable, reproducible retention across a broad pH window, which is essential when methods require changes in buffer strength, ionic strength, or pH to resolve close variants.

In biopharmaceutical analysis, many critical quality attributes (CQAs) appear as small charge differences rather than large mass differences. PolyCAT A is widely selected for charge heterogeneity work because it can separate acidic/basic variants and modified forms while maintaining clean peak shapes and high recovery.

Because the support is polymeric, the column is typically tolerant to higher salt, a range of aqueous buffers, and repeated runs where ionic strength is ramped for elution. This makes it useful for both analytical QC and fraction collection when preparative purity is needed.

PolyETHYL A is a hydrophobic interaction phase tailored for proteins and peptides where preserving native structure is important. In HIC, retention increases in high salt and decreases as salt is reduced—this allows separations to be performed under relatively mild, aqueous conditions.

The ethyl ligand provides a balanced hydrophobic surface that can resolve very small differences in exposed hydrophobic patches. This is valuable in bioprocess work where aggregates, clipped species, and subtle conformational variants may differ only slightly in hydrophobicity.

PolyETHYL A is often used as part of multidimensional workflows (for example, ion‑exchange followed by HIC) and can be applied for both analytical profiling and preparative purification, depending on the format.

PolyHYDROXYETHYL A is a workhorse hydrophilic interaction (HILIC) phase chosen for polar compounds and biomolecules. HILIC typically uses high organic (often acetonitrile) with a controlled aqueous component, which can improve MS sensitivity and provide strong retention for polar species that are weakly retained on reversed‑phase columns.

A key advantage is versatility: besides HILIC separations, the phase can be operated under fully aqueous conditions where it behaves more like a size‑exclusion style separation for certain applications (method dependent). This makes it useful for labs that need one platform for multiple workflows.

It is widely adopted in proteomics and biopolymer analysis—useful for peptide mapping and digest fractionation, oligonucleotides, and complex matrices where desalting/cleanup is needed before downstream analysis.

PolySULFOETHYL A is a strong cation‑exchange phase optimized for peptides rather than intact proteins. Its capacity and selectivity enable high‑resolution fractionation of complex peptide mixtures, which is why it is widely used as a first‑dimension separation in 2D‑LC and LC‑MS proteomics.

Peptides often differ by only one or two charges across the working pH range; PolySULFOETHYL A is designed to exploit those differences to separate peptides into cleaner fractions, improving depth of coverage and reducing ion suppression in MS.

Beyond proteomics, it is valuable for synthetic peptide QC and purification, and for targeted enrichment or isolation of specific peptide classes (depending on method conditions).

PolyMETHYL A provides a controlled HIC surface that is often chosen when you want careful tuning of hydrophobic interactions. The methyl ligand offers a different selectivity window compared with ethyl and propyl phases, helping resolve variants that may co‑elute on other HIC chemistries.

It is frequently used in biopharmaceutical characterization where small structural changes can shift hydrophobic behavior—such as clipped species, glycoform-related surface changes, or formulation‑induced conformational shifts.

Because HIC is performed under aqueous, salt‑driven conditions, PolyMETHYL A can be integrated into workflows where maintaining protein integrity and recovery is important.

PolyWAX LP is a weak anion‑exchange phase designed for analytes that are negatively charged under working conditions—such as nucleic acids, acidic proteins, and polyanionic species. Weak anion exchange can provide gentle interaction control, enabling high recovery and flexible method tuning with pH and ionic strength.

It is commonly used for oligonucleotide and PCR‑product related separations, as well as for proteomics prefractionation where acidic peptides or biomolecular subsets are enriched prior to downstream analysis.

The column is also useful for separating mixtures of acidic solutes and biomolecules in research and QC settings where robust, repeatable ionic selectivity is required.

PolyGLYCOPLEX A is a neutral, high‑capacity HILIC material optimized for carbohydrate and glycan separations. Because many glycan species are highly polar and structurally similar, a dedicated glycan HILIC phase is often the most reliable way to achieve consistent, high‑resolution profiles.

It is widely used for glycosylation analysis of biopharmaceuticals, separating both neutral and charged (e.g., sialylated) glycans. The phase is compatible with MS‑friendly mobile phases and can be used for native glycans or for labeled glycans (such as fluorescent derivatives), depending on the lab’s workflow.

For method development, the column is valued for reproducibility, strong retention, and the ability to resolve subtle structural differences that affect glycan polarity and interaction with the stationary phase.

PolyPROPYL A is a high‑selectivity hydrophobic interaction phase that typically provides stronger retention than methyl or ethyl HIC chemistries. This makes it valuable when complex protein mixtures require additional retention and separation space to resolve close species.

It is frequently selected in multidimensional purification workflows where strong HIC selectivity improves fraction purity, and in research applications such as polypeptide purification and challenging biomolecule mixtures where hydrophobic differences drive separation.

The polymeric construction supports robust operation under repeated high‑salt conditions, making it suitable for routine purification runs as well as investigative method development.